2.1 Cell Structure
Prokaryotic Cells
Microscopes
Magnification - How much bigger an image appears compared to the original object.
Resolution - Ability to produce an image of fine detail clearly.
Magnification = image size/actual size
Types of microscopes
Optical Microscopes
Laser scanning microscopes
Electron Microscopes
Transmission electron microscopes
Scanning electron microscopes
Relatively Cheap
Easy to use
Portable
Can study whole living specimens
Use laser light to scan object point by point and displayed on a computer screen.
Allow magnification of up to x1500 and x2000
Resolution is limited, they cannot magnify any higher without giving a clear image
High resolution and show high contrast
Depth selectivity and can focus on structures at different depths
Used in medical professions
Chemically fixed by being dehydrated and stained.
Beam of electrons passes through the specimen
Large and very expensive
Need a great deal of skill and training to use
Ultra structure of eukaryotic cells
Nucleus, Nuclear Envelope and Nucleolus
Endoplasmic Reticulum
Nucleolus don't have a membrane, contain RNA.
Chromatin genetic material consisting of DNA . When cell's not dividing chromatin is spread out. When cell's about to divide chromatin coils tightly into chromosomes.
Nucleus surrounded by double membrane - nuclear envelope. Some regions have inner and outer membrane fuse together to allow ribosomes through.
The nucleus is the control centre of the cell, stores the organism's genome, transmits genetic information, provides the instructions for protein synthesis.
Rough ER
System of membranes containing fluid filled cavities coated in ribosomes.
Smooth ER
System of membranes containing fluid filled cavities but have no ribosomes.
Intracellular transport system cisternae form channels for transporting substances to other parts of cell.
Contains enzymes that catalyse reactions involved with lipid metabolism:
-Synthesis of cholestrol
-Synthesis of lipids
-Synthesis of steroid hormones
Involved in absorption, synthesis and transport of lipids.
Golgi Apparatus
Stack of membrane bound flattened sacs. Secretory valves bring materials to and from the Golgi
Proteins are modified by: -adding sugar molecules to make glycoproteins -adding lipid molecules to make lipoproteins -being folded into their 3D shape
Proteins packaged into vesicles that are pinched off then:
-Stored in the cell
-Moved to plasma membrane to be incorporated in the plasma membrane or exported outside the cell
Mitochondria
Spherical, rod-shaped or branched. Surrounded by two membranes with fluid filled space between. Inner fluid is a fluid filled matrix.
Mitochondria are site of ATP production. Self-replicating so if cell needs more energy they can increase. Abundant in cells where much metabolic activity takes place.
Chloroplasts
Vacuole
Lysosomes
Ribosomes
Centrioles
Cytoskeleton
Cellulose Cell Wall
Large organelles 4-10 um. Only found in plant cells and surrounded by double membrane or envelope. Inner membrane is continuous with stacks of flattened membrane sacs - thylakoids which contain chlorophyll. Stack of thylakoid called granum. Fluid filled matrix is stroma.
Chloroplasts are site of photosynthesis
Surrounded by membrane called tonoplast, contains fluid
Only plant cells have vacuole. Filled with water and solutes and maintains cell stability - when full pushes against cell wall, making cell turgid.
Small bags formed from Golgi, surrounded by a single membrane. Contains digestive enzymes and are abundant in phagocytic cells.
Engulf old cell organelles and foreign matter, digest them and return digested components to cell for reuse.
Small spherical organelles, 20 nm diameter. Made of ribosomal RNA and produced in the nucleolus as two subunits which when passed through nuclear envelope combine.
Two bundles of microtubules are right angles. Made of tubulin protein subunits to form a cylinder.
Synthesising proteins bound to RER and site of assembly of proteins when free in cytoplasm.
Spindle of cell division forms from centrioles
Network of protein structures in cytoplasm: Made of micro-filaments, internediate filaments, cyndrical microtubules and cycloskeletal motor proteins.
Give support and mechanical strength, allow cell movement, help substances and organelles move through cytoplasm.
Provide strength and support, maintain the plant's shape and are permeable to allow solutions to pass through.
Cell wall of plants on the outside of plasma membrane - bundles of cellulose fibres.
Proteins
- mRNA copy instructions for insulin made in nucleus
- mRNA leaves nucleus through nuclear pore.
- mRNA attaches to ribosome of RET. Ribosome reads instructions to assemble protein.
- Insulin molecules pinched off in vesicles travel towards Golgi.
- Vesicle fuses with Golgi
- Golgi processes and packages insulin molecules ready for release.
- Packages insulin molecules pinched off in vesicles from Golgi move towards plasma membrane
- Vesicle fuses with plasma membrane
- Plasma membrane opens to release insulin molecules
Have a plasma membrane
Cytoplasm
Ribosomes for assembling amino acids into proteins
DNA and RNA
Smaller than eukar
no centrioles and less well developed cytoskeleton
do not have a nucleus
don't have membrane bound organelles
have a wall made of peptidoglycan and not cellulose
have smaller ribosomes
have naked DNA floating free in cytoplasm
Divide by binary fission and not by mitosis