2.1 Cell Structure

Prokaryotic Cells

Microscopes

Magnification - How much bigger an image appears compared to the original object.

Resolution - Ability to produce an image of fine detail clearly.

Magnification = image size/actual size

Types of microscopes

Optical Microscopes

Laser scanning microscopes

Electron Microscopes

Transmission electron microscopes

Scanning electron microscopes

Relatively Cheap

Easy to use

Portable

Can study whole living specimens

Use laser light to scan object point by point and displayed on a computer screen.

Allow magnification of up to x1500 and x2000

Resolution is limited, they cannot magnify any higher without giving a clear image

High resolution and show high contrast

Depth selectivity and can focus on structures at different depths

Used in medical professions

Chemically fixed by being dehydrated and stained.

Beam of electrons passes through the specimen

Large and very expensive

Need a great deal of skill and training to use

Ultra structure of eukaryotic cells

Nucleus, Nuclear Envelope and Nucleolus

Endoplasmic Reticulum

Nucleolus don't have a membrane, contain RNA.

Chromatin genetic material consisting of DNA . When cell's not dividing chromatin is spread out. When cell's about to divide chromatin coils tightly into chromosomes.

Nucleus surrounded by double membrane - nuclear envelope. Some regions have inner and outer membrane fuse together to allow ribosomes through.

The nucleus is the control centre of the cell, stores the organism's genome, transmits genetic information, provides the instructions for protein synthesis.

Rough ER

System of membranes containing fluid filled cavities coated in ribosomes.

Smooth ER

System of membranes containing fluid filled cavities but have no ribosomes.

Intracellular transport system cisternae form channels for transporting substances to other parts of cell.

Contains enzymes that catalyse reactions involved with lipid metabolism:
-Synthesis of cholestrol
-Synthesis of lipids
-Synthesis of steroid hormones
Involved in absorption, synthesis and transport of lipids.

Golgi Apparatus

Stack of membrane bound flattened sacs. Secretory valves bring materials to and from the Golgi

Proteins are modified by: -adding sugar molecules to make glycoproteins -adding lipid molecules to make lipoproteins -being folded into their 3D shape

Proteins packaged into vesicles that are pinched off then:
-Stored in the cell
-Moved to plasma membrane to be incorporated in the plasma membrane or exported outside the cell

Mitochondria

Spherical, rod-shaped or branched. Surrounded by two membranes with fluid filled space between. Inner fluid is a fluid filled matrix.

Mitochondria are site of ATP production. Self-replicating so if cell needs more energy they can increase. Abundant in cells where much metabolic activity takes place.

Chloroplasts

Vacuole

Lysosomes

Ribosomes

Centrioles

Cytoskeleton

Cellulose Cell Wall

Large organelles 4-10 um. Only found in plant cells and surrounded by double membrane or envelope. Inner membrane is continuous with stacks of flattened membrane sacs - thylakoids which contain chlorophyll. Stack of thylakoid called granum. Fluid filled matrix is stroma.

Chloroplasts are site of photosynthesis

Surrounded by membrane called tonoplast, contains fluid

Only plant cells have vacuole. Filled with water and solutes and maintains cell stability - when full pushes against cell wall, making cell turgid.

Small bags formed from Golgi, surrounded by a single membrane. Contains digestive enzymes and are abundant in phagocytic cells.

Engulf old cell organelles and foreign matter, digest them and return digested components to cell for reuse.

Small spherical organelles, 20 nm diameter. Made of ribosomal RNA and produced in the nucleolus as two subunits which when passed through nuclear envelope combine.

Two bundles of microtubules are right angles. Made of tubulin protein subunits to form a cylinder.

Synthesising proteins bound to RER and site of assembly of proteins when free in cytoplasm.

Spindle of cell division forms from centrioles

Network of protein structures in cytoplasm: Made of micro-filaments, internediate filaments, cyndrical microtubules and cycloskeletal motor proteins.

Give support and mechanical strength, allow cell movement, help substances and organelles move through cytoplasm.

Provide strength and support, maintain the plant's shape and are permeable to allow solutions to pass through.

Cell wall of plants on the outside of plasma membrane - bundles of cellulose fibres.

Proteins

  1. mRNA copy instructions for insulin made in nucleus
  1. mRNA leaves nucleus through nuclear pore.
  1. mRNA attaches to ribosome of RET. Ribosome reads instructions to assemble protein.
  1. Insulin molecules pinched off in vesicles travel towards Golgi.
  1. Vesicle fuses with Golgi
  1. Golgi processes and packages insulin molecules ready for release.
  1. Packages insulin molecules pinched off in vesicles from Golgi move towards plasma membrane
  1. Vesicle fuses with plasma membrane
  1. Plasma membrane opens to release insulin molecules

Have a plasma membrane

Cytoplasm

Ribosomes for assembling amino acids into proteins

DNA and RNA

Smaller than eukar

no centrioles and less well developed cytoskeleton

do not have a nucleus

don't have membrane bound organelles

have a wall made of peptidoglycan and not cellulose

have smaller ribosomes

have naked DNA floating free in cytoplasm

Divide by binary fission and not by mitosis