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Agarose Gel Electrophoresis - Coggle Diagram
Agarose Gel Electrophoresis
Agarose Gel Preparation
Gel Rig Preparation
Insert comb into slots at ends of tray
Pour molten 1% agarose gel into tray
Allow to solidify 20 min.
450 mL 1x TBE: pour small amount onto surface of gel
Pull comb out and straight out gel
Lift tray out of rig and rotate 90 degrees
Fill both sides with 1x TBE buffer until gel covered by 2 mm buffer
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DNA samples
uncut plasmid DNA
cut plasmid DNA
cut genomic DNA
λHindIII ladder or 1KB ladder
uncut genomic DNA
Set P20 micropipette to 20μl.
Load DNA samples and one of the two ladders
Slide lid onto tank until power supply leads attached to electrodes.
Running the Gel
Turn on power pack. Set voltage to 115-120V.
Let gel run until dye front moves 5 cm from wells
After complete: Turn off power. Slide lid off tank.
Drain buffer into tank. Slide gel into weigh boat.
View gel through using blue box