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RT-PCR- detects the presence of viral nucleic acids in nasopharyngeal swab…
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RT-LAMP (Reverse Transcriptase- Loop mediated isothermal amplification)- one-step nucleic acid amplification method to multiply specific sequences of RNA of the coronavirus. Here, the RNA is first made into cDNA (copy DNA) by the usual reverse transcription. Then, the DNA is amplified by the LAMP technique
Advantage
technology is done at 65 degrees C, where the DNA amplification is done at a constant temperature (isothermal), so that expensive thermal cycler is not required.
quantity of DNA amplified in the LAMP technology is hundred thousand times more than that is taking place in PCR
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