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Animal cell culture : - Coggle Diagram
Animal cell culture :
Application
Model system
Toxicity study
Drug screening and development
3D cell culture and bioprinting
Bioproduct
Genetic counselling
Gene therapy
Advantages
Research tool
Mimic
in vivo
cell
Highly selective
continous supply
Reduce the use of animal
Easy to manipulate
Disadvantages
Difficult to maintain aseptic environment
High cost
Genetic variation
Limited passage number
Cross-contamination
Low expression of protein marker
Cell maintenance
Media
Natural
Artificial
Add serum or not?
Physicochemical
pH
Humidity
Temperature
Oxygen
Carbon dioxide
Cell density
Hayflick's phenomenon
Cell counting
Hemocytometer
Cell viability
Type
Primary
Anchorage-dependent
Anchorage-independent
Cell line
Finite
Continous
Procedure
Facility
Biosafety cabinet
Process
Aseptic technique
Personnel
Filtering and autoclaving
Hood regulation
Maintain aseptic environment
Waste management