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Clostridium Species, Anaerobic, gram-positive rods,
non-spore forming…
Clostridium Species
C. perfringens
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gram positive, spore-forming bacillus
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requires collection of: three blood cultures, stool and bowel contents, or bowel tissue
specimens can be plated to: anaerobic blood agar, selective or differential media (EYA medium)
biochemical reactions: gelatin hydrolysis positive, lecithinase positive
C. botulinum
produces a potent neurotoxin that is responsible for botulism - flaccid paralysis of respiratory and other muscle groups
gram positive, spore forming bacillus
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acceptable specimens: feces, enema fluid, gastric aspirates, vomitus, tissue, exudates, post-mortem speciemns
biochemical reactions: beta-hemolytic, indole negative, lecithinase negative
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acquired from: ingestion (foodborne), colonization of gastrointestinal (GI) tract (infant botulism), infected wounds
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C. tetani
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gram positive, spore-forming bacillus
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Anaerobic, gram-positive rods,
non-spore forming bacilli
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