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PROCESSING OF ANAEROBIC CULTURES - Coggle Diagram

- - - - examine individual colonies for distinctive morphologies
        
        gram stain and subculture to BAP and CHOC
        
        gram-negative add kanomycin, vancomycin, or colistin disk in first quadrant, nitrate disk in second quadrant
        
        gram-positive cocci add nitrite and SPS
        
        gram-positive rod add nitrate
        
        incubate anaerobically 37°C for 24 to 48 hours
        
        AEROTOLERANCE TEST: subculture organisms to a CHOC plate, incubate 37°C for 24 to 48 hours in 5% CO2 to detect slow growing aerobes