Please enable JavaScript.
Coggle requires JavaScript to display documents.
Seed coat sculpturing in Halophila - Coggle Diagram
Seed coat sculpturing in
Halophila
Abstract
external morphology of the seed coats of selected
Halophila
Fruiting plants of
Halophila becarii, H. ovalis, Halophila sp.
were collected (Malaysia) and
Halophila stipulacea
(Mauritus)
seeds extracted fixed in 2.5% glutaradehyde
fixed seeds- washed in 0.1M Sodium cacodylate, buffer at 4C for 10 minutes
seeds were dehydrated in acetone series of 35%, 50%, 75% and 100%
specimens were examined with JEOL 6400 scanning electron microscope
Introduction
monocotyledonous aquatic plants
with leaves, roots, rhizomes
comprise less than 0.02% of angiosperm flora
seagrasses flower- develop fruit, produce seeds
seeds-nutrients storage, germination
3 Main types of functional seeds in seagrasses
i. seeds with hard pericarp
ii. seeds with a membranous coat, without a distinct dormancy period
iii. seeds which neither have a seed coat nor a dormancy period
Materials and Methods
H. beccarii, H. decipiens, H. ovalis, Halophila sp.
were collected
seeds extracted from mature fruits were fixed in 2.5% glutaradehyde
fixed seeds were then washed in 0.1M sodium cacodylate buffer
washing procedure repeated 3 times
seeds then dehydrated in graded alcohol series of 35%,50%, 75%, 95% and 100%
followed by in acetone, dried in Baltec 030 critical-point dryer
specimens were examined with a JEOL 6400 scanning electron microscope
Observations and Discussion
Halophila seeds
sub-globose,bluntly beaked at both ends
Halophila beccarii, H. decipiens, H. ovalis
have reticulate testa or seed coats
seed coat surface of
H. beccarii
has reticulation with a pale short brown peg in the center of the reticulation
seed coat of
H. decipiens
has hexagonal reticulation without peg at center and rough within reticulation
H. ovalis
, the surface of the seed recovering has a distinct isometric or rectangular reticulation and some microsculptures
seed of
Halophila sp.
is sub-globose, seed coat without reticulation but verrucose in appearance
Halophila stipulacea
seed sub-globular, reticulated seed coat surface