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Enzymes - Coggle Diagram
Enzymes
Phosphorlyase
Test Tube 4
1.5 mL 0.01 M G1P
1 drop 0.2% starch
Iodine Test -
2 mL boiled phosphorylase
Iodine Test
Test Tube 5
1.5 mL 0.01 M G1P
0.5 mL 0.2 M KH2PO4
1 drop 0.2% starch
Iodine Test -
2 mL fresh phosphorylase
Iodine Test
Test Tube 3
1.5 mL 0.01 M G1P
1 drop 0.2% starch
Iodine Test -
2 mL fresh phosphorylase
Iodine Test
Test Tube 6
0.5 mL 0.2 M KH2PO4
1.5 mL 0.2% statch
Iodine Test +
2 mL boiled phosphorylase
Iodine Test
Test Tube 2
1.5 mL 0.01 M G1P
1 drop 0.2% starch
Iodine Test -
2 mL fresh phosphorylase
Iodine Test
Test Tube 7
0.5 mL 0.2 M KH2PO4
1.5 mL 0.2% starch
Iodine Test +
2 mL fresh phosphorylase
Iodine Test
Test Tube 1
1.5 mL 0.01 M glucose
1 drop 0.2% starch
Iodine Test -
2 mL fresh phosphorylase
Iodine Test
Test Tube 8
4 mL Phosphorylase
Boil 10 min.
Salivary Amylase
Test Tubes #6-10
Test Tube 6: 2 mL of 1% amylase
37°C water bath 5 min.
Test Tube 8: 2 mL of 5% amylase
37°C water bath 5 min.
Test Tube 7: 2 mL of 2% amylase
37°C water bath 5 min.
Test Tube 9: 2 mL of 10% amylase
37°C water bath 5 min.
Test Tube 10: 2 mL water
37°C water bath 5 min.
Test Tubes #11-15
Test Tube 11: 1% Starch Suspension + 2 mL McIlvaine's buffer
37°C water bath 5 min.
+Test Tube 6
Iodine Test. 1 min, Until -
Test Tube 14: 1% Starch Suspension + 2 mL McIlvaine's buffer
37°C water bath 5 min.
+Test Tube 9
Iodine Test. 5 sec., Until -
Test Tube 12: 1% Starch Suspension + 2 mL McIlvaine's buffer
37°C water bath 5 min.
+Test Tube 7
Iodine Test. 30 sec., Until -
Test Tube 13: 1% Starch Suspension + 2 mL McIlvaine's buffer
37°C water bath 5 min.
+Test Tube 8
Iodine Test. 15 sec., Until -
Test Tube 15: 1% Starch Suspension + 2 mL McIlvaine's buffer
37°C water bath 5 min.
+Test Tube 10
Iodine Test. 30 sec., #14 time
Test Tubes #1-5
Test Tube 1: 2 mL 1% Salivary Amylase
1 Drop Iodine- Spot Plat
1 Drop Test Tube- Spot Plate/Iodine
+4 mL Benedict's Solution
Test Tube 2: 2 mL 2% Salivary Amylase
+4 mL Benedict's Solution
Test Tube 3: 2 mL 5% Salivary Amylase
+4 mL Benedict's Solution
Test Tube 4: 2 mL 10% Salivary Amylase
+4 mL Benedict's Solution
Test Tube 5: 2 mL 1% Starch Solution
1 Drop: Iodine Test
Remaining: Benedict's Test
Test Tubes #16-20
Test Tube 17: +4 mL Benedict's Solution
+2 mL Test Tube 12
Boil 5 min.
Test Tube 18: +4 mL Benedict's Solution
+2 mL Test Tube 13
Boil 5 min.
Test Tube 16: +4 mL Benedict's Solution
+2 mL Test Tube 11
Boil 5 min.
Test Tube 19: +4 mL Benedict's Solution
+2 mL Test Tube 14
Boil 5 min.
Test Tube 20: +4 mL Benedict's Solution
+2 mL Test Tube 15
Boil 5 min.