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Fluorometry - Coggle Diagram
Fluorometry
ADVANTAGES:
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This will measure the intensity of fluorescence, and sample concentration.
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It is more precise than the absorption process because both emission and excitation wavelength are characteristics.
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DISADVANTAGES:
The fluorescence method is that it only analyzed fluorescent molecules, not all.
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PRINCIPLE: In UV-Visible spectroscopy, the excitation wavelength is measured but with FLUOROMETRY the emission wavelength is measured.
When a substance is subjected to radiation of excitation wavelength, the electrons in the atom reach singlet excited state. They then transit from singlet excited state to singlet ground state by emission of radiation with a specific wavelength. This emitted radiation is measured for analysis.
COMMON USES: To accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality. Example, paternity test and genetic chromosome testing
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