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Atomic absorption, Osmometry, Electrochemistry - Coggle Diagram
Atomic absorption
Advantages
sensitive and precise
used to measure
concentration of trace metals
that are not easily excited.
Disadvantages
he inability of the flame to dissociated
samples into free metals.
ex phosphate may interfere with calcium analysis by formation of calcium phosphate.
ionization of atoms following dissociation by The
flame, which can be decreased by reducing the
flame temperature.
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Used to measure concentration by detecting
the absorption of electrophoresis radiatiom
by atoms
rather than by molecultes
Osmometry
Used to determine the molecule
mass which depends on colligative
propertirs remaining
that the number of dissolved molecules
is the only factor that alters the properties of a solution
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Disadvantages
- Boiling point elevation is most useful for clinical samples because proteins will coagulate causing gross changes in sample composition
- Volatile gases if present will increase the vapor pressure of solvent
Advantages
- simple to use requires very little knowledge or scientific back growth to operate systems and achieve good results .
- the most widely referenced and practiced technique for osmolity testing have be en commercially available for over 50 years.
Test ran on the osmometer
urine osmolatity test
checked in urine,
chloride
glucose
potassium
sodium
urea
3 major types of osmometers
- freezing point osmometrr
- vapor pressure osmometrr
- membrane osmometrr
Electrochemistry
Basis for many types of analysis are used in
clinical lab including potentiometers, amperometry, coulometry, and polarography.
the 2 cells involved are galactic and electrolytic cells
2 half cells and a salt bridge which can be a piece of filter paper saturated with electrolytes
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Disadvantages
- narrow or limited temperature range
- cross sensitivity of other gases
Advantages
- Simplicity, low cost and speed.
- ability to access unique reactions and transformations that are not possible by other techniques
