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Protein Supplementation Does Not Affect Myogenic Adaptations to Resistance…
Protein Supplementation Does Not Affect Myogenic Adaptations to Resistance Training
Praticipants (58)
(protein blend [PB]
Age 24.1 +- 0.6
n=22
whey protein [WP]
Age 24.6 +- 1.0
n=15
maltodextrin placebo [MDP]
Age 25.2 +- 1.1
n=17
habitual protein intake for participants was ~1.3 gIkg -1Id-1,
randomized (20 per group) to the placebo (MDP), whey (WP), or blend (PB) isocaloric treatments as previously described
Information concerning treatment compliance, dietary intake, and strength testing.
Study design
frozen muscle samples necessary for immunohistochemical analysis
three nonconsecutive days of exercise fa- miliarization and before one-repetition maximum (1RM) strength testing.
body com- position, a muscle biopsy, and an isometric and isokinetic strength test of the thigh as we have previously described in the initial clinical trial
participants reported to the UTMB Alumni Field House for familiarization/ testing before beginning 12 wk of training.
After 12 wk of training, participants were retested exactly 3 d after the final exercise session of the training program.
For the posttesting, participants reported to the Institute for Translational Science Clinical Research Center at the same time in the morning as for the pretraining study day to repeat the same laboratory tests and sample collection.
Immediately after each workout, under direct observation of the study personnel, the participants ingested either the placebo bever- age or one of the protein supplements to which they were assigned.
On the four resting (nonexercise) days each week, the participants ingested the placebo or supplement one time between meals
RET. After familiarization and 1-RM strength testing, participants began a 12-wk whole-body progressive RET program as we have previously described in the initial clinical trial
Exercise sessions were performed on nonconsecutive days, three times weekly, with four rest days per week, under supervision of qualified personal trainers.
familiarization and the 1-RM testing were conducted after the muscle biopsy.
A percuta- neous biopsy sample of the VL muscle was performed using a 5-mm Bergstro ̈m biopsy needle with suction, under sterile procedure and local anesthesia (1% lidocaine).
RNA
Immunohistochemistry
Samples were removed from the cork at j25-C in a ThermoFisherCryostat (Fisher Scientific HM 525X) where they were cut in 7-Km cross sections.
RNA concentration. Total RNA was isolated by ho- mogenizing 10–20 mg of tissue with a handheld homoge- nizing dispenser (T10 Basic Ultra Turrax, IKA, Wilmington, NC) in 1 mL of Tri reagent
Separated into
aqueous phase using 0.2 mL of chloroform
subsequently precipitated from ~475 KL of aqueous phase using 0.5 mL of isopropanol.
Total RNA was quantified by measuring the total volume of the aqueous phase. RNA was washed twice with 1 mL of 75% ethanol, air-dried, and suspended in a known amount of nuclease-free water.