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Microscopy ( optical instrument uses lens to produce magnified images of…
Microscopy ( optical instrument uses lens to produce magnified images of small objects
Parts
Head or Body tube
connects the eyepiece to the objective lenses
Ocular or eyepiece
type of lens that is attached to a variety of optical devices
Stage
The flat platform that supports the slides
Arm
Structural element that connects the head of the microscope to the base - what a person holds when they are carrying the microscope
Nosepiece
This circular structure is where the different objective lenses are screwed in
Condenser
gathers light from the microscope light source and concentrates it into a cone of light
Coarse focus
rough (and basic) focus knob on the microscope
Iris diaphragm
located under the stage and is an apparatus that can be adjusted to vary the intensity, and size, of the cone of light that is projected through the slide
Fine focus
knob used to fine tune the focus of a specimen in conjunction with the coarse focus
substage
device that supports mirrors and other attachments under the common stage of a compound microscope
base
The bottom of the microscope - what the microscope stands on.
Stage clips
hold the slides in place
Objective lenses
10X( low power objective)
cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens.
aligning microscope
40X (high-dry objective)
useful for observing fine detail
striations in skeletal muscle
the arrangement of Haversian systems in compact bone
types of nerve cells in the retina
4X( scanning objective)
shortest objective and is useful for getting a general overview of a slide
100X (high-oil objective)
used to increase the resolving power of a microscope
terms
contrast
the difference in light intensity between the image and the adjacent background relative to the overall background intensity.
Field of view
maximum area visible when looking through the microscope eyepiece (eyepiece FOV) or scientific camera (camera FOV)
resolution
indicates the level of detail actually observed in the specimen.
Field diameter
the number of millimeters or micrometers you will see in your whole field of view when looking into the eyepiece lens.
resolving power
smallest detail that a microscope can resolve when imaging a specimen; it is a function of the design of the instrument and the properties of the light used in image formation.
orientation of image
change in optics of microscopes lenses
magnification
increase in apparent size of object