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Near-term Grand Challenge, Grand plan - Coggle Diagram
Near-term Grand Challenge
Grand plan
Establishing molecular biology supply center in the middle east
PCR polymerase
Taq
Pfu
KOD
improving PCR polymerases
hot start
salt tolerance
PCR inhibitor tolerance
DNA modifying enzymes
gp5
Ligase
more enzymes
PNK
protein modifying enzymes
SUMO protease
TEV
RNA dependent polymerase
MMLV-RT
improving MMLV-RT
new MMLV-RT to make better cDNA library
improve thermostabiltiy
Future enzymes
Assembling kit
One-step RT-qPCR kit
assembling one-step RT-qPCR kit
production of enzymes
performance of enzymes
optimizing the conditions
performance of the kit with patient samples
improving one-step RT-qPCR kit
engineering proteins
Antibody
MMLV-RT (SSII, SSIII)
improve Taq activity
RT-LAMP kit
assembling RT-LAMP with patent-free eznymes
assembling RT-LAMP with BR3 Po and its mutants
developing the lysis buffer
designing the primers for dieases
improving the RT-LAMP
thermostalibity of MMLV-RT
salt tolerance of MMLV-RT
salt tolerance of Bst
primer design
testing primers for COVID-19
designing primers for other diseases
choosing proper dye to judge by naked eyes
RPA kit
assembling RPA kit with patent-free enzymes
enzyme production
optimizing condition
kit performance
improving RPA kit
Fen1 as a reporting enzymes