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Lecture 9: transcription - Coggle Diagram
Lecture 9: transcription
Properties of mRNA
ssDNA, but a lot of intermolecular base pairing
Hairpin (A-U)
one of two strand of DNA used as template
top? bottom? depends on gene
can happen on opposite directions
mRNA on template strand, same sequence as coding strand
RNA polymerase
where does energy for RNA synthesis come from?
RNA+ribonucleotide triphosphate
hydrolysis ^ and release lots of energy
product: PPi
used to power strand separation and translocation
Prokaryotes VS. Eukaryotes
Prokaryotes: One RNA polymerase
Eukaryotes: RNA Polymerase I, RNA Polymerase II, RNA Polymerase III
Steps
Initiation
How to decide which strand to use and where to start? Promoter