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CBC: Blood film review, sampling, quant. data intro. - Coggle Diagram
CBC: Blood film review, sampling, quant. data intro.
Complete Blood Count (CBC)
minimum database
sampling
whole free-flowing venous blood
21-23g needle to reduce hemolysis
vacutainer
appropriate sample type
appropriate sample volume
otherwise,
artifacts
dilutional changes
morphology changes
for anti-coagulated
EDTA
mammalian CBCs
purple/lavender top
chelates Ca
hemolyzes bird/reptile blood cells
sodium heparin
green top
binds and potentiates AT-III
bird and reptile CBC
citrate
generates plasma
light blue top
coagulation testing
quantitative data
erythrogram
RBC mass
HCT/PCV
% of blood volume occupied by RBCs
increase = erythrocytosis
decrease = anemia
HCT is calculated
artifacts
inadequate sample volume decrease PCV
prolonged storage increase PCV
RBC count
unit = # * 10^6/ul
artifacts
agglutination decreases count
hemolysis
may increases count through ghost cells (in vivo disease process)
may decrease count (sampling error)
Hgb
RBC size
hemoglobin
spectrophotometric measurement
artifacts
sample turbidity
Heinz bodies
hemolysis
lipemia
RBC morphology
RBC indices
mean cell volume (average size of RBC)
analyzing anemias
microcytic
normocytic
macrocytic
MCHC (mean cell hemoglobin concentration
leukogram
thrombogram
proteins
qualitative data
RBC morphology
parasitism
WBC morphology
blood film review
1-2 smears made ASAP
dry rapidly
staining
types
Ramnowsky stain
aka Diff-Quik
Wright-Giemsa
new methylene blue
stains...
heinz bodies
RNA reticulocytes
impermanent
process
alcohol = fixation
metachromasia
acidic dye = pinken basic components
proteins
granules
Hgb
basic dye = acidic components blue
nucleic acids
combined effect of acidic and basic dyes
DNA
RNA
mast cell granules
