7.0 10^5/mL human FBs and,
7.0 10^5/mL human ECs
with or without 3.5 10^5/mL human PCs,
suspended in a *solution comprising
2.2mL of 3.5 mg/mL of rat tail type I collagen
150 mL of FBS ,
290 mL of10X pH re- constitution buffer (0.05M NaOH, 2.2% NaHCO3, and 200mM HEPES), and
290 mLof 10 ·HAM-F12 medium (Gibco) with subsequent storage at 4˚C to avoid gelation.