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Membrane contact sites - Coggle Diagram

- - - - can occur between most organelle pairs, but ER is a crucial hub
        
        4 types of proteins involved
        
        Structural (tethers and spacers)
        
        Functional
        
        Regulatory
        
        Sorter
- - - - Reduced to Fe2+ by ferrireductase prior to exit from endosome via DMT1 channel
        
        Iron incorporation step into the haem occurs in the mitochondria
        
        Studies show that endosome and mitochondria come close together and form transient contact sites "Kiss and Run". Using EM and fluorescent studies
        
        Studies using TfR that cannot release iron (lock-hTf) contact sites still occur, but last for longer
        
        Protein linker still unknown
- - - - PH domain
        
        Binds Arf1 and PI(4)P on the Golgi
      - FFAT motif (FF-acidic tract)
        
        Allows OSBP to bind VAP on ER via its MSP domain
      - ORD domain
        
        Binds either cholesterol or PI(4)P
        
        Extract lipid from PI(4)P rich TGN, due to the presence of PI4 kinase and transfers it to the ER where concentration of PI(4)P is low due to the presence of the PI(4)P phosphatase sac 1
        
        PI(4)P gradient gives directionality
        
        PI(4)P hydrolysis provides energy to transfer cholesterol against it's concentration gradient