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TECHINIQUE IN MOLECULAR BIOLOGY :microscope: (STUDY PROMOTER FUNCTION AND…
TECHINIQUE IN MOLECULAR BIOLOGY :microscope:
EMSA
tell if protein bind to DNA
process
run on the gel, migrate towards the positive end
DNAse FOOTPRINTING
CHiP
PCR
NORTHERN BLOT
process
isolate RNA
denature and separate RNA by electrophoresis
transfer to membrane; fix, hybridize
radiolabeled antisense probe
WESTERN BLOT
SOUTHERN BLOT
IN VITRO RUN OFF ASSAY
STUDY PROMOTER FUNCTION AND ACTIVITY
5' DELETION SERIES
LINKER SCANNING
use reporter gene to facilitate the analysis of gene promoter activity