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UMUC1-LV CRISPR KO (FOXA1 (WB (Shows knockdown in sg1 (hi-GFP). Need to…
UMUC1-LV CRISPR KO
FOXA1
WB
Shows knockdown in sg1 (hi-GFP). Need to repeat for low-GFP
Repeat WB
RT-qPCR
Doesn't show knockdown in RT-qPCR
Is there something wrong with b-actin gene expression between cells?
Try different primer
GATA3
WB
Shows knockdown in (lo-GFP). Need to repeat for low-GFP
Repeat WB
RT-qPCR
Doesn't show knockdown in RT-qPCR
Is there something wrong with b-actin gene expression between cells?
Try different primer
NPAS2
WB
Knockdown seen only in ambgood LV
Repeat WB
RT-qPCR
Shows knockdown overall in all gRNAs