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CHROMATOGRAPHY (Definition (Collective term form a set of laboratory…

- - - - Flowing past a thin layer of powder on a solid support
      - Substances less attracted to solid or more soluble in liquid move faster
      - Move further up the plate by time that the process been stopped by taking plate out of liquis having larger Retention Factor (Rf)
        
        Substance are soluble in liquid if Rf close to 1
        
        Substance are insoluble in liquid if Rf close to 0
    - - Purpose: Separate a homogeneous mixture into its individual components
      - Uses: Separation & Identification
      - Chromatography paper: Stationary phase
      - Solvent: Mobile phase
  - - - Partition chromatography
      - Adsorption/ Liquid-solid chromatography
      - Ion exchange chromatography
      - Size exclusion/ gel chromatography
    - - Compounds separated by injecting sample mixture onto the column.
      - It's instrument consist of reservoir of mobile phase, pump, injector, separation column and detector.
      - Form of liquid used to separate compounds that are dissolved in solutions.
      - Uses
        
        Used for chemistry and biochemistry research analyzing complex mixture, purifying chemical compounds, developing process for synthesizing chemical compounds, isolating natural products or predicting physical properties.
        
        Used in quality control to ensure the purify of raw materials, to control and improve process yields, to quantify assays of final products or to evaluate product stability and monitor degradation.
        
        Used for analyzing air and water pollutants.