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Nucleic Acids (Hershey and Chase (They prepared two strains of T2, one…
Nucleic Acids
Hershey and Chase
Used the virus T2 to infect cells of the bacterium E.coli to investigate whether genes were made of DNA or protein
T2 virus consists of only DNA inside a protein coat. DNA contains phosphorus but not sulphur, and protein contains sulphur but not phosphorus.
They prepared two strains of T2, one having its DNA radioactively labelled with 32P and the other with 35S.
Results found that E.coli injects its DNA into the bacterium injects DNA into the bacteria. This gave strong evidence for genes being composed of DNA rather than protein.
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Base sequences in DNA
Sanger sequencing
Sanger developed a method of base sequencing. It was based on nucleotides of didexoyribonucleic acid. These contain dideoxyribose instead of deoxyribose, so have no -OH group on the carbon atom 3.
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Gene expression
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Only in prokaryotes can translation begin before transciption is finished,
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