PhD thesis committee meeting presentation
Slide 1
Slide 2
Year one
In this slide I will give a short outline of the presentation
As could be seen from the title - focus is on Proteostasis in aging and stress.
Actually there is a whole range of question of interest - Starting from
BIG questions:
How important is the proteostasis in the process of aging? ; What comes first - changes in proteostasis that causes aging, or aging causing changes in proteostasis?
Medium rank questions
How important is proteasome in proteostasis network? ; Are there any changes in the behaviour of proteasome during aging? Changes in the behaviour of proteasome upon exposure to stress? - What is the importance of this to Aging?
Highly specific questions
What are the interactors of 19s subunit of proteasome in physiological conditions?
Hopefully as my PhD progresses, my experiments will enable me to address questions higher and higher up this list.
Aim (goal) of the 1st year of my PhD:
Develop a method for detection of proteasome interactors.
Why is that important?
It will allow me to address some of the questions mentioned on the previous slide ,
Establishing the technique(proximity dependent biotinylation)
APEX
Validating the technique
BioID
Validating the technique
Apply it to model system and answering specific questions about proteasome
Apply it to model system and answering specific questions about mitochondria proteome
Intro(PN, PN in aging, conventional methods of PPI detection, methods of PPI detection based on proximity biotinylation.
BioID
BioID Validation
BioID Application
APEX
APEX Validation
APEX Application
Future Plans
Slide 3
Improvements to the techniques
Future questions to address
Proteostase and AGING
Slide 4
Classical vs Proximity biotinylation dependent techniques of PPI detection
Slide 5
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