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JC (Transcription factors confer multi-lineage engraftment (COMPARISSON OF…
JC
Transcription factors confer multi-lineage engraftment
COMPARISSON OF MULTILINEAGE CELLS AND CD45 CELLS IN FACTOR MINUS 1 STUDY
COMMON TFs EXPRESSED IN hiPSCs, hESCs, CFU
FACS ANALYSIS OF FACTOR MINUS 1 STUDY
COMPARISSON OF ENGRAFTMENT IN BM LIBRARY, 7TFs % 5TFs
RT PCR FOR 7 TFs EXPRESSED IN THE ENGRAFTED DIFFERENTIATED CELLS
SCREENING OF THE 7 TFS IN DIFFERENT LINEAGES PROVING 5 IS EXPRESSED.
COMPARISSON OF MULTILINEAGE CONTRIBUTION WITH hESCs ND CB.
VALIDATION OF SELF RENEWAL, AND REPOPULATION ABILITY
FACS ANALYSIS MULTI-LINEAGE CONTRIBUTION WITH CB AND hESCs
ROLE OF EHT CULTURE HAS BEEN FOUND THAT ITS BECAUSE THE REPROGRAMMING TAKES PLACE IN EHT GOING CELLS NOT ENDOTHELIAL CELLS
LIMITING DILUTION ASSAY
TP CELLS Vs SP CELLS
In vivo screening of transcription factors
EMBRIOID BODY FORMATION
SCREENING FOR TRANSCRIPTION FACTORS
MICROARRAY ANALYSIS HE VS FL-HSCs 12 TFs
POOLED UP WITH TFs USED FOR PREVIOUS SUCH STUDIES 26 TFS IN TOTAL.
DO EHT CULTURE
QRT PCR DATA FOR ENDOTHELIAL MARKERS AND HSC MARKERS
FACS ANALYSIS FOR CD45+ CELLS
VECTOR DESIGNING
DOX ON pINDUCER WITH GFP MARKER AS A LABEL
IN VITRO SCREENING BY FACS
IN VITRO SCREENING BY FLORESCENCE MICROSCOPY
IN VIVO SCREENING BY ENGRAPHTING INTRAFEMORALLY IN TO NSG MICE
FACS ANALYSIS OF CD45+ CELLS FROM BONE MARROW AND THYMUS
BLOOD CHIMERISM ANALYSIS OF CD45+
SORTING HEMOGENIC ENDOTHELIAL CELLS
CULTURE IPCs
Characterization of differentiated cells
NEUTROPHILS
MYELOPEROXIDASE ASSAY WITH AND WITHOUT PMA STIMULATION
ERYTHROCYTES
QRT PCR FORFETAL AND ADULT BETA GLOBIN MEASUREMENT
NUCLEAR STAINING FACS FOR NUCLEATED AND ENUCLEATED RBCs
B AND T CELLS FUNTION
MEASURING IgM ND IgG WITH AND WITHOUT STIMULTION