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microguide.com (lab safety (Emergency Procedures (Small fires should be…

- - - - Microbiological procedures
        
        personal habit
        
        nothing in moth
        
        wash hands for 20 mins
        
        contaminated
        
        finished
        
        disinfectant your desk
        
        before start
        
        finished
        
        if spill culture
        
        disposing used items properly
        
        labelling
        
        Label all cultures and solutions properly with your name or initials, date, course / lab section, and experiment information. The experiment information may include the composition of the solution, identity of the culture, or the experiment number.
        
        Biosafety Guidelines:
        
        Biosafety levels are determined by the National Institutes of Health and the Centers for Disease Control.
        
        Biosafety Level 1
        
        well-characterized agents not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.
        
        open bench tops using standard microbiological practices. Special containment equipment or facility design is neither required nor generally used
        
        Biosafety Level 2
        
        is suitable for work involving agents of moderate potential hazard to personnel and the environment.
        
        Precautions for dealing with body fluids - use Biosafety Level 2
    - - Because microorganisms are so small, large populations are generally needed in order to monitor microbial activity. Aseptic techniques help ensure that only one type of microorganism is present in a container (pure culture). These methods also ensure that the microorganisms do not escape from the container, contaminating the laboratory, and possibly causing disease.
    - - Items contaminated with microorganisms or body fluids must be autoclaved before disposal or washing.
      - Needles and syringes must be thrown into the red sharps container. Syringes without needles must be disposed of in the sharps container.
      - A special cart is designated in the laboratory for items that require autoclaving, including:
        
        Glassware including flasks, beakers and other containers. Remove all marks from the glass with ethanol and place the items in the cart. Do not throw cultures down the drain. Place the containers and their contents in the cart.
        
        Glass tubes are placed in baskets set on an angle so that the contents do not spill. Remove marks before disposal using ethanol.
        
        Any items that are contaminated with culture or bodily fluids.
        
        Plastic Petri plates and other disposable plastic items used to culture or contain microorganisms must be placed in the red biohazard bags for autoclaving prior to disposal.
      - Chemicals must be disposed of properly. Some chemicals must be placed in special containers for disposal to prevent environmental contamination. Your laboratory instructor will inform you about the chemicals you are using.
      - Never pick up broken glass with your hands; use a broom. Dispose of broken glass in the broken glass container. Disposable glass items that are not contaminated with culture must be placed in the broken glass container - even when they are not broken.
      - Paper towels should be thrown in the ordinary trash, unless they are contaminated with culture.
  - - - Prevent contact with the skin by wearing gloves and a lab coat.
      - Minimize hazards due to the formation of dangerous aerosols and splashing by wearing protective eye gear
      - Limit exposure to chemical fumes by working in a chemical fume hood.
      - Properly dispose of chemicals that pose environmental hazards.
    - - Set up a Bunsen burner in the center of your lab bench in an area free of overhanging materials. Never leave a lighted Bunsen burner unattended. Keep work area clear.
      - Keep water away from electrical equipment.
      - Some procedures in the lab involve physical hazards and require special precautions, for example:
        
        Working with hot items, either from the autoclave or heated in the Bunsen burner requires protection of your hands. Wear mitts or handle the hot item with tongs.
      - Using a sonicator, or sonic dismembranator, requires wearing protective ear coverings.
      - When working with a UV light source, wear coated UV-protective safety glasses.
      - Protective eye coverings are required any time that aerosols might be generated or splashing might occur.
  - - - A fire extinguisher is ready in each laboratory.
    - - For heat burns, chill the burned area with cold water or ice and notify the instructor.
- - - - Agar Culture
      - Broth Culture
      - medical Definition of wet mount. : a glass slide holding a specimen suspended in a drop of liquid (as water) for microscopic examination; also : a specimen mounted in this way.
  - - - Plate Streaking
      - Broth Transfer
      - Pipette Transfer
      - Flaming the Loop