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glycoysis FOR IRENE CHECHI (pyruvate dehydrogenase kinase pdk (pyruvate…
glycoysis FOR IRENE CHECHI
pyruvate dehydrogenase kinase pdk
pyruvate hydrogenase pyruvate to acetyl coa
enters to krebs by
pdk deactivates the pyruvate dehydrogenase
pdk is activated by hif 1a during hypoxia
exp 1
metabolome analysis
high level of pyruvate is produced in lt hscS
atp level is also less
glucose uptake is same
exp 2
LT HSCs REDUCED
2dg INHIBITOR
inhibited glucose uptake
EXP 3
NO RATE CHANGE
MITOCHNDRIAL RESPIRATION INHIBITED
EXP 4
DEFICIENCY SHOWED DECREASE IN GLYCOLYTIC ENZYMES AND GLUT 1
REDUCED ACTIVITY OF LDH
HIF 1a KO
LACTATE RELEASED IS REDUCED WHICH IS RELEASED BY LTHSCs
CHECK THE ACTIVITY OF PDK CORELATES WITH HIF1a
TO CHECK HIF 1a KO AND WILD PDK WAS TRANSFECTED
IN KO THE PDH PHOSPHORYLATED AND GLYCOLYSIS AND LDH ACTIVITY INCREASED LTHSC NO. AND TRANSPLANTA
P16 GENE IS UPREGULATED SO THE APOPTOSIS IS MORE THEN IT MAY DUE TO THE LACK OF PDK LEADS TO THE MITOCHONDRIAL RESPIRATION AND ROS PRODUCTION
MULTYCOLOUR FLOW CYTOMETRY
BUT THERE WAS LESS PYRONIN FRACTION (LESS RNA) AND
PDK 2&4 DELETED LTHSCS NO DIFFERENCE
FROM EXP 123 WE CAN SUGEST IT DEPENDS IN GLYCOLYIS
EXP 5