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HATCHERY MANAGEMENT (P. MONODON) (LARVAE FEEDING (PROTOZOEA :sports…
HATCHERY MANAGEMENT
(
P. MONODON
)
BROOSTOCK
:checkered_flag: select large productive, healthy, free disease
:checkered_flag: size ♀ TL>28cm, BW>217g
:checkered_flag: size ♂ TL>21cm, BW>70g
:checkered_flag: bright color, clean body/gill, undamaged appendages
SPAWN &
HACHING
:warning: techniques used promote production
(high quality, disease free
ablate intermoult (fully hard shell) ♀ 5 day after transportation
cut eye with hot pincers
disinfect area with Pure Liquid Povidone (PVP)
after ablation
pre@ post-moult ♀~ ablate after 1week
1st spawn ~3-7day after ablate ~5pm ~stage 4 ovary
1 ♀ /spawn tank (500L)
water filter through ~active carbon ~cartridge filter(1-5micron) ~preferable UV
clean seawater 10-30ppm EDTA & 0.05-0.1 ppm treflan
PROBIOTICS
work better than antibiotic
uses of antibiotic are:
:skull_and_crossbones: often ineffective
:skull_and_crossbones: expensive/dangerous/illegal
:skull_and_crossbones: slow growth & low immunity
LARVAE
STOCKING
:star: 100-150 nauplii/L
WATER EXCHANGE
:star: no water exchanged, but add water daily (4-6 day zoea stages)
:star: 10-30 %/day ( 4-6 day mysis period)
:star: 30-50%/day (PL1-PL5)
:star: >50%/day (PL6-PL15)
TANK
:star: 50-75% capacity, clean, disinfected, filtered seawater
:star: 28-30°C~ 30-35ppt~ 7.8-8.2 pH~
:star: <0.1ppm NH3( Ammonia)~ <0.1ppm NO2 (Nitrite)
LARVAE FEEDING
PROTOZOEA
:sports_medal: 50K cell/ml
Skeletonem
a @
Chaetoceros
:sports_medal: 10K cell/ml
Tetraselmis
:sports_medal: micro-encapsulated feed (50-125u) : 16ug/larva/day. increased daily by 20%
MYSIS
:warning: 100-200 rotifer (
Brachionus plicatilis
)/day
:warning: 20-50 Artemia nauplii/day
:warning: micro-encapsulated feed (125-250u) :16ug/larva/day & increased daily 20%
NAUPLII
:!: no feeding (egg yolk)
PL
(P1-P6)
:pen: 100-200 Artemia nauplii/ PL/day
:pen: micro-encapsulated feed (250-350u) :16ug/larva/day & increased daily 20%
HEALTH
ASSESSMENT
Larvae
inspected twice/day
stage, health, activity, behaviour, feed/faeces, body
detailed microscopic examination
PCR
(screen for viral)
once (2-3day b4 harvest)
*twice (nauplii/PL5)
PL QUALITY TEST
:syringe: ~gross,microscopic exam
~stress,vibrio test, PCR
:syringe: perform on each tank (PL10-13)
:syringe: 2-3days b4 harvest the PL (PL15)
LIVE/PRESEVED
ALGAE
DIATOM
MICROALGAE
good for early stage (Z1-M)
perfect nutrion
self suspended in water
enhance water quality (NH3,NO2)
maintain shade
natural & helpful antibiotic
enrich nutritional value of artemia