Please enable JavaScript.
Coggle requires JavaScript to display documents.
CLONING MMP20 GENE INTO pWH1520 TO MAKE ENAMELYSIN :smiley: (2**This…
CLONING MMP20
GENE INTO
pWH1520 TO MAKE
ENAMELYSIN :smiley:
1**What is it?
Covers teeth affecting color and thickness
Hard crystals made by proteases
Enamelysin is a protease
MMP20 gene codes for it
2**This study?
Strategy
Insert MMP20 into pWH1520
Restriction enzymes (BamHI and SmaI)
PCR to amplify MMP20
Gel electrophoresis
T4 DNA ligase
Transformation
Screening
3 more items...
Vector Diagram
Making enamelysin
Future studies
Is this protease enough to make enamel?
3**If there is enamelysin made?
Future studies
Is enamelysin enough to make enamel?
use it with other proteases to make enamel