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Controls (types of controls (Technical (types (Positive (prevent false…

- - - - prove that your samples has been normalized
        
        certain change in signal is due to change in condition, not loading difference
        
        load housekeeping proteins, expect same level of all
  - - - Positive
        
        prevent false negative
        
        don't get signal when should
        
        add isobutanol directly instead of culture supernatant
        
        enzyme in the assay can do the work
        
        Or as a known amount
      - Negative
        
        prevent false positive
        
        get a signal when shouldn't
        
        Add water instead of culture supernatant
        
        oxidized MTT is not reduced when there is no isobutanol
        
        Or as a blank
    - - get true positive and true negative
  - - - generate mutant
    - - Positive
        
        use strain that you known will produce isobutanol
        
        bacteria can be engineered, and can produce isobutanol
      - Negative
        
        use strain without over-expression pathway
        
        isobutanol measurement is dependent on over-expression