BRUCELLOSIS - LABORATORY DIAGNOSIS (Milk Sample (Competitive ELISA (by…
BRUCELLOSIS - LABORATORY DIAGNOSIS
Facultative intracellular organisms
Sheeps and goats
Polymerase Chain Reaction (PCR)
detect Brucellae in tissues
for international trade
for identifying infected herds or flocks
milk ring test
For monitoring infections
Rose-Bengal plate test
for screening test
Complement-Fixation test (CFT)
For confirmatory test in individual animals
for reliable screening and confirmatory test
by using monoclonal bodies - to detect all immunoglobin classes
to differentiate infected animals from S19-vaccinated cattle
Serum-Agglutination test (SAT)
a tube agglutination test which may lead to false-negative results
for detecting non-agglutinating antibodies not detected by the SAT
Gram Negative, non-acid fast, stain red by Stamp's modification of the Ziehl-Neelsen's method, coccobacilli and arranged singly.
Isolation of Brucellae sp.
Culture on Columbia agar + 5% serum
Samples: uterine discharge, aborted fetuses, udder secretions, lymphnodes, reproductive organs.
Castaneda medium for isolation from blood and other body fluids or milk
removed aseptically with sterile instruments, prepared by removal of extraneous material, cut into small pieces, and macerated using a 'Stomacher' or tissue grinder with small amount PBS, before being inoculated.
Collected cleanly after washing and drying whole udder and disinfecting the teats, about 10 to 20 ml collected where the first stream is discarded. milk is centrifuged and the cream and deposit are spread on solid selective medium.
Vaginal swab taken after abortion or parturition, then streaked on to solid media.
Cheese- cultured onmedia where enrichment culture is advised. homogenised before culture after macerated with appropriate volume of PBS.
all samples should be cooled immediately; frozen if not be cultured immediately.