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U21 NT APEX2-FLAG transfection --buffered in 25 mM HEPES pH 6.8 (plate A -…
U21 NT APEX2-FLAG transfection --buffered in 25 mM HEPES pH 6.8 (plate A - 171120)
no drug control in buffered media (plate B - 171124)
cells look good, not seeing the cell death like in the drugged wells - fed RPMI + HEPES (plate B - 171128)
cells look good - going to stop the HEPES and split/drug (171130)
no drug control - no longer adding HEPES (plate C - 171130)
fed/drugged 1X PMA + 1X hydro (plate D5 - 171204)
fed/drugged PMA + TSA (plate D5 - 171206)
2 ml RPMI + PMA + hydro + 25 mM HEPES (plate E5 - 171213)
2 ml RPMI + PMA + hydro (plate D5 - 171213)
fed/drugged 1X PMA + 0.5X TSA (plate C6 - 171204)
fed/drugged PMA + hydro (plate C6 - 171206)
1X PMA + 1X hydro (plate C5 - 171130)
fed/drugged (plate C5 - 171202)
fed/drugged PMA + TSA (plate C5 - 171206)
infection doesn't look great but it's the best of the NT infections so I decided to split these cells. One of the wells just got RPMI (plate C5 - 171209)
fed RPMI + HEPES (plate E2 - 171209)
1X PMA + 0.5X TSA (plate C4 - 171130)
fed/drugged (plate C4 - 171202)
fed/drugged PMA + hydro (plate C4 - 171206)
1X PMA + 1X hydro in buffered media (plate B - 171124)
lots of cell death - added ~6,000 ND10 cells (plate B - 171126)
still mostly dead cells - spiked in ~10,000 ND10 cells (plate B 171202)
these cells all died :cry:
1X hydro + 0.5X TSA in buffered media (plate B - 171124)
lots of cell death - added ~6,000 ND10 cells (plate B - 171126)
fed with RPMI + 1X PMA (bc I had incorrectly drugged at first) - stopped adding HEPES - think this might be causing the cell death (plate B - 171128)
still mostly dead cells - spiked in ~10,000 ND10 cells (plate B 171202)
these cells all died :cry: