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Binge-Like Consumption of a Palatable Food Accelerates Habitual Control of…
Binge-Like Consumption of a Palatable Food Accelerates Habitual Control of Behavior and Is Dependent on Activation of the Dorsolateral Striatum
Experiments
1
--> 36 rats
--> operant chambers
- magazin food dispension
- pellet or sugar solution
- retractable lever next it
Design
pretreatment phase
--> 3 groups of 12
--> free access to regular food and water for 5 weeks
--> 2 groups
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continuous access
--> always access to condensed milk !
--> chow, water and milk
restricted access (2 hour binge eating!!!)
--> been shown to increase food consumption
---> access to condensed milk 2 hours a day after weighting
------> chow water and milk
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instrumental training !!
--> 3 day before start food restriction 15g chow (maintain body weight
--> half conditioned to respond to pellets half to milk
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day 2
--> levers introduced
--> until 60 reinforcements (VI schedule 15 to 60s) or 75 minutes passed
------> VI advanced only if 60 reinforcements met otherwise more training
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c-Fos immunohistochemistry
--> anesthesises
--> coronal brain slices stained
--> c-fos counted light microscope
results
Calorie intake
--> food restriction = signify more intake than always group and control
--> all signif
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devaluation in extinction test
--> main effect: devalued responded les (lever presse) except RESTRICTED!!
--> means if they had access to the food they had before in the devalued training ( pellets and then pellets again or milk then milk again) they pressed lever less often :3 !!
-----> expect restricted !!!
CFOS !!
CFOS IR DLS (for habit)
--> more in restriction than control and contious
--> control and continue no difference
what about brain regions that send input from DLS ?
--> infralymbic cortex = for habit
--> prelymbic = for goal
infralymbic cortex
--> restricted = more cfos than control (but not continuous)
---> type 2 error cause continue should be different from control then too ...
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2
--> experiment 1 showed increased activity of DLS and associated cortex
--> cortex = strong glumtamatic input on DLS ,
WHAT IF we disrupt that ?
--> will it make the act of making the preferred food less rewarding more effective in preventing binge eating ?
--> ampa receptor antagonist CNQX into DLS
design
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devaluation training with AMPA antagonist (and dopamine antagonist)
--> devaluing or non devaluing and either saline or AMPA antagonist
--> if pellet = milk
--> if milk = pellet
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after extinction = put into cage ad libitum to 30 grams of preferred if pellet then pelletst or of milk then milk
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blabla
habits vs goal
goal
--> what you think the outcome of an action might be
AND
--> the value you assign to that outcome :3 !!
--> dorsal medial striatum
habit
--> reaction to well learned stimulus ---> not directly influenced by modifying the value of the outcome of an action !! #--> exposure to amphetamine , alcohol etc enhances habit learning --> dorsal lateral striatum
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Why ?
Does long term exposure or restriction to food accelerate/ chance the formation of habits vs animals without this exposure?
(similar with exposure to amphetamine, alc, etc)
--> measured by outcome devaluation :3 !! #
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then assessed if these changes in dorsal striatum regions are necessary for changes in habit learning