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U21 C-term APEX2-FLAG transfection -- buffered in 25 mM HEPES pH 6.8…
U21 C-term APEX2-FLAG transfection -- buffered in 25 mM HEPES pH 6.8 (plate A - 171120)
1X PMA + 1X hydro in buffered media (plate B - 171124)
lots of cell death - added ~6,000 ND10 cells (plate B - 171126)
still mostly dead cells - spiked in ~10,000 ND10 cells (plate B 171202)
all these cells died :cry:
1X hydro + 0.5X TSA in buffered media (plate B - 171124)
lots of cell death - added ~6,000 ND10 cells (plate B - 171126)
fed with RPMI + 1X PMA (bc I had incorrectly drugged at first) - stopped adding HEPES - think this might be causing the cell death (plate B - 171128)
still mostly dead cells -
spiked in ~10,000 ND10 cells (plate B 171202)
all these cells died :cry:
no drug control in buffered media (plate B - 171124)
cells look good, not seeing the cell death like in the drugged wells - fed RPMI + HEPES (plate B - 171128)
cells look good - going to stop the HEPES and split/drug (171130)
1X PMA + 0.5X TSA (plate C1 - 171130)
fed/drugged (plate C - 171202)
fed/drugged PMA + hydro (plate C1 - 171206)
1X PMA + 1X hydro (plate C2 - 171130)
fed/drugged (plate C - 171202)
moved to a T25 flask, fed/drugged PMA + hydro (T25C - 171206)
mixed 1:1 with ND10 cells + PMA + hydro (T25C - 171212)
no drug control - no longer adding HEPES (plate C1 - 171130)
1X PMA + 1X hydro
fed/drugged PMA + hydro (plate D4 - 171206)
1X PMA + 0.5X TSA
fed/drugged PMA + hydro (plate C3 - 171206)