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METHODS (RNA Isolation (Lyse cells to obtain RNA from the cytosol,…
METHODS
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RNA Isolation
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Use a gDNA Eliminator Spin Column and Rneasy Spin column to discard genomic DNA from the sample and obtain purified RNA
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cDNA Synthesis
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Incubate the Samples at Room Temperature for 10 min, 42°C for 50 min, 70°C for 15 min and store the samples at -20°C
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