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Elizabethkingia.spp (Bacterial strains collection (in vitro (Sequencing -…

- - - - broth micro dilution
        (gold standard confirm) # #
        
        Levofloxacin
        
        Cirofloxacin
        
        Tigecycline
    - - resistance mechanism
        
        Efflux pump?
        
        Efflux pump inhibitor(EPI)
        
        PAbetaN
        
        MIC>512 後續不易執行
        
        NMP
        
        初步結果加NMP可使MIC下降
        
        2 more items...
- - - - CME<已建立方法篩選>
      - GOB,BlaB<已建立方法篩選>
  - - - Levofloxacin / Ciprofloxacin
        
        phenomenon
        
        Recent reference
        
        QRDR，gyrA ParC
        
        antimicrobial susceptibilty
        
        susceptible
        resistant
        intermediate
        
        E.spp
        
        DNA gyrase, subunit A,(type II topoisomerase)
        
        PCR/Sequcing<請煜瑄完成後續>
        
        Result analysis<待基龍米克斯定序結果分析>
        
        合成Primer(已完成)
        
        這些抗藥菌株基因分型的結果
        
        Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations
        
        gyrB
        
        DNA gyrase, subunit B (type II topoisomerase)
        
        5 more items...
        
        ParC(DNA topoisomerase IV subunit A)
        
        合成Primer(已完成)，共有二組。一組for E.anohpelis and e.miricola，一組for E.menigoseptica 找不到更conserved sequence
        
        初步發現，其ParC gene no mutation in QRDR region such as AA site 78 80 84 #
        
        ParE DNA topoisomerase subunit B
        
        ParE(DNA topoisomerase IV subunit B)
        
        Novel mutations were found at five different codons and comprised Glu453-Gly His461-Tyr), Ala498-Thr , Val512-Gly, and Ser518-Cys.
        
        we found one change in the amino acid sequence of GyrB of strain 197 (Ser-494 to Thr), and a Gln substitution was observed in ParE Pro-424 of strain 112.