Please enable JavaScript.

Coggle requires JavaScript to display documents.

Bacterial cultivation, preservation and inactivation (Inactivation useful…

- - - - From fixed smear, arbitrary number of fields counted
      - Doesnt account for viability of cell
    - - Make 10 fold serial dilutions, spread fixed volume on each plate and incubate
      - Count colonies on plates. Viable organisms in suspension=CFU
    - - Opacity tubes. Compare to McFarland Standards
      - Spectrometry/Optical density
      - Quick, but no info if viable
- - - - Defined: known amounts of known chemicals
      - Complex: hyrolysates, extracts, etc
    - - Selective media limits growth of unwanted microbes
      - Differential allows differentiation between different microbes
      - A medium can be both
- - - - Moist heat more effective than dry heat
  - - - Surfactants
        
        Acid-anionic detergens
        
        Cationic detergents
      - Phenols an phenolics
      - Halogens
        
        Chlorine
        
        Iodine
      - Alcohols
      - Heavy metals
        
        Oligodynamic action -> denature proteins by altering disulphide bonds
        
        Silver
        
        Mercury
        
        Copper
- - - - Require oxygen but only small amounts
    - - Obligate anaerobes
      - Aerotolerant anaerobes
      - Facultative anaerobes (can grow with or without O2)