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Cultivation, Preservation and inactivation (Bacterial Inactivation…

- - - - 10 fold serial dilations
      - Count colonies on plates
    - - Direct fixed smear
        
        Arbitrary number of fields counted
      - Counting chamber
    - - Opacity tubes
      - Quick
- - - - Exact Chem. composition not known
- - - - Require O but in small amounts
    - - Obligate
      - Aerotolerant anaerobes
      - Facultative anaerobes
- - - - modified live vaccines for control studies
  - - - Stored as ampoules in dark
      - Not all microorganisms
- - - - inanimate objects
    - - Living tissue