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Recombinant protein expression (Yeast producer strain (decreased protein…
Recombinant protein expression
Chassis strain
auxotroph
heterothallic
https://www.yeastgenome.org/locus/S000002386
do not form pseudohyphae
https://doi.org/10.1111/j.1574-6976.2001.tb00573.x
Yeast producer strain
Protein overexpression cassette(s)
Promoter
Constitutive
Inducible
Improved proteins folding
Chaperones
protein disulfide isomerase overexpression (like PDI1)
https://doi.org/10.1038/nbt0494-381
Improved heterologous protein secretion
Protease genes knock-out
Proper post-translational modification (humanized PTM)
Mannosylation
https://doi.org/10.1007/s12033-019-00164-8
Mnn2p
Mnn11p
Och1p
mnn1
N- linked glycosylation genes
https://doi.org/10.1016/j.copbio.2007.09.001
OCH1
ALG3
ALG11
decreased protein degradation
PEP4
PRB1
Decrease contamination - deletion of secreted protein genes
Decrease degradation after secretion
Yapsine proteases deletion
yps1Δ YLR120C
yps6Δ YIR039C
yps3Δ YLR121C
yps7Δ YDR349C
mkc7Δ YDR144C
vacuolar proteases
https://doi.org/10.4161/cl.28023
vps10
vps4
vps8
vps13
vps35
vps36