Ecosystems of Homewood Campus Part VI: Analyze DNA Sequences and Identify…
Ecosystems of Homewood Campus Part VI: Analyze DNA Sequences and Identify Bacteria
Part A: Download Your Bacteria's DNA Sequence From the Johns Hopkins DNA Services Core Facility
Scroll to top of screen.
Pick view chromat trace.
The reliable portion of the DNA won't be crossed out. Copy this part and save it to a new file in Microsoft Word.
Take a screenshot of the chromatogram and paste it into a Word file and save it.
Click all and find our sequence in the Label column.
Now view the DNA sequence and decide if it's usable. Compare the sequence to DNA sequences in worldwide databases.
Data then View Folders; pick the folder for this year
Determine the identity of your bacterial sample
Username is pearlman and password is study
Part E: Research your bacterial species or genus
Retrieve the full text, including figures, of one article. This article must be from a peer reviewed scientific journal and must be a research article, not a review article.
Find a second resource to use for the presentation.
Use a database to find a journal article about your bacterial species.
Get approval from the TA for the species and journal article. Save everything you need.
Look up information about your bacterial species.
Part F: Prepare to Present Your Data to Your Classmates
Include general information about the bacteria such as the name, well-known relatives, any interesting chemical products produced by it.
Presentation must include data from your experiment such as the soil sample take, the appearance of the culture along with a photograph. A chromatogram, BLAST data for your top match, and GenBank record for your top match.
Include information about your journal article (time to work on this in class next week). What are the authors trying to prove and did they succeed?
Explain any raw data you show.
Hand in a copy of the journal article.
Can only be 12 minutes.
Include information from the metagenomics lab exercise.