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mFISH Probe Sets and their Applications (APPLICATIONS (Whole chromosome…
mFISH Probe Sets
and
their Applications
ABOUT
Used for a precise description of complex chromosomal rearrangement
1996 - Routine application on human chromosomes began
Established to characterize marker chromosomes identified in conventional banding.
Simultaneous use of at least three different ligands or fluorochromes for the specific labelling of DNA.
PROBE SETS
YAC
YAC-based chromosome bar code has been created for chromosome 12
Can achieve up to 400 bands
Region-specific Yeast Artificial Chromosome
IPM-FISH
Resolution: 400 bands per haploid karyotype
SCAN
Spectral Color Banding
8 microdissection librabries were created along chromosome 10
Aim : To obtain a banding pattern similar to the GTG-banding at the 300 band level
MCB
High-resolution Multicolor-Banding
Based on overlapping microdissection libraries producing fluorescence profiles along the human chromosomes
Harlequin-FISH
Lowest resolution - 80-80 bands per haploid human karyotype.
Consists of flow-sorted gibbon chromosomes, which are labeled with three different fluorochromes
mBAND
Allows the differentiation of chromosome-region-specific areas at the band and sub band level
Resolution: 550 bands per haploid karyotype
Based on the same aforementioned principle, but slightly different microdissection derived probes than MCB
CENTROMERE and/or LOCUS SPECIFIC PROBE SETS
acroM-FISH & acro-cenM-FISH
Characterization of the short arms
Characterization of centromeric regions of the of the acrocentric chromosomes
cenM-FISH
Allow simultaneous characterization of all human centromeres
Use labeled centromeric satellite DNA as probes
Distinguishes all centromeric regions apart from the evolutionary highly conserved ones on chromosomes 13 and 21
subcenM-FISH
Subcentromere - specific multicolor FISH
Specifically paints a chromosomal region rarely characterize by other available FISH or mFISH probes
subtelomeric probes
To stain different human telomeres
APPLICATIONS
Rx-FISH
Applied in clinical, leukimia and solid tumor cytogenetics as well as in Zoo-FISH studies
MCB-probe
Able to resolve complex aberrations in comparison to other techniques
Most flexible resolution : 400-800 bands per haploid karyotype
IPM FISH
Connecting banding cytogenetics and M-FISH
cenM-FISH
Characterization of small supernumerary marker chromosomes
Whole chromosome painting
Search for cryptic rearrangement
Characterization of marker chromosomes
Confirmation, refinement and characterization of translocation
acroM-FISH
Characterize small SMC derived from acrocentric chromosome
subcenM-FISH
Characterization of the euchromatic content of small supernumerary marker chromosome
Characterization of rearranged chromosome