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Cell Staining (Bacterial Identification/Characteristics (colony morphology…
Cell Staining
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Gram Stain
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based on differences in the membrane layers of bacteria (thickness of the peptidoglycin layer varies which gives it different properties)
procedure
take a small amount of cells from the streaked petri dish/sample using a sterile toothpick and mix into the water in the circle; spread it around so it dries faster
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KOH Test
can indicate if the bacteria is gram negative or gram positive because gram negative cells will lyse in the solution, releasing their cell contents and the DNA will make it stringy
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