Please enable JavaScript.
Coggle requires JavaScript to display documents.
Lab Skills (Dilutions (Ratio (for example, 1:4 dilution is 1 part solutionโฆ
Lab Skills
Dilutions
Ratio (for example, 1:4 dilution is 1 part solution and 4 parts water)
-
-
-
in our lab, we made dilutions of enzyme concentration to create a standard curve by measuring the absorbance at known concentrations
Pipetting
-
-
How to use it
-
- attach the disposable tip
- depress the plunger to the first stop (will be lower for larger volumes)
- insert into the solution and allow the plunger to move up slowly
- place the tip on the side of the tube for empty tubes or right about the meniscus for tubes with sample already in it
- fully depress the plunger then remove; eject tip into waste bin
in our lab, we pipetted different amounts of blue dye with micropipetters and again with serological pipettes to compare our accuracy using a spectrophotometer
Spectrophotometer
-
want to measure a specific molecule so use a blank which contains all of the molecules in the sample EXCEPT the one you wish to observe
be careful to remove smudges from the cuvette and avoid placing labels in the way of the light stream
-
-