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Microscope Lab (Tips on Using Compound Microscopes (Oil Immersion lens…

- - - - being with the condenser as close to the slide stage as possible then move it down as the resolution improves
  - - - adjust from 4x to 40x then rotate the lenses so that it's in between the 40x and 100x then add the drop of oil to the slide so that it doesn't touch any other lense
- - - - when the yeast is engulfed by the protozoa it moves into the food vacuoles where the pH is lower so as it is digested the Congo Red turns to purple
        
        can treat the protozoa with gluteraldehyde which fixes them, allowing us to see digestion of the yeast in various stages
- - - - can use oil immersion
      - inverts and reverses the image
      - How to Prepare Slides
        
        Depression slides are thicker and have a circular well usually for larger specimens; don't need a coveslip
        
        Flat slides are used for smaller specimens and do need a coverslip
        
        prepare by placing a drop of solution on the slide, holding the cover at an angle, and gently easing it down while ensuring that no air bubbles remain in the slide
    - - to determine the current resolution, multiple this value by the 10x due to the ocular lense