Please enable JavaScript.
Coggle requires JavaScript to display documents.
Electrotaxis (Paramenters (Serum (Growth Factors (GF) (EGF (epithelial…
Electrotaxis
Paramenters
-
Serum
Growth Factors (GF)
-
-
-
-
-
The polarisation response is dependent on the of serum.
Serum free: no significant perpendicular reorientation up to 200 mV/mm
Cells in medium with 10% FBS: reorientation occurred at 100 mV/mm
Adding single growth factors to serum-free medium restored field induced responses. However, combinations of growth factors can cause higher directness than individual growth factors
The extent of EF-induced directness depends on growth factors concentration (dose-dependence) and is specific to each individual GF. At higher concentrations the directness of migration decreased or virtually disappeared (receptor internalisation and degradation)
-
EF importance
No EF - random orientation
The applied EF adds a vector to cell movement. Induces receptor redistribution and cathodal/anodal facing membranes
Serum/GF importance
Serum free - no significant orientation/migration
The polarization/migration is depend on the serum
In the presence of serum/GF the threshold of migration decreases (<100 mV/mm)
Corneal injuries
-
Instantaneously induce a laterally oriented DC field resulting from the collapsed trans-corneal epithelial potential difference
-
-
Affecting cell migration, proliferation and nerve growth at cornea wounds in vivo
-
-
-
- Directed elongation and guidance motility by EFs (galvanotaxis) has been shown in keratocytes
- Drugs that affect ion transport have been used to increase or decrease the EF strength - regulating wound healing
At non wounded state:
- Electrical potential differences: tens of mV across tens of µm
- Electric voltage gradients: hundreds to thousands mV/mm
Injury :warning: Na+ and K+ leak out the wound. result: ionic current, with the wound as cathode
Experimental 