Measuring Transpiration (PRACTICAL)

A POTOMETER can be used to estimate transpiration rate

Measure Water uptake

1) Cut a plant shoot underwater

2) Put the potometer in the water

All air is trapped

3) Keep the capillary tube in the water

4) Dry the leaves, then shut the tap

5) Let 1 air bubble out and record the distance moved

Environmental Conditions affect the rate

Light Intensity

Use a lamp to increase rate

Put the potometer in a cupboard to decrease the rate

Temperature
(warm and cold room)

Increase in temp, increase in rate

Decrease in temp, decrease in rate

Humidity (spray water into a plastic bag)

Should decrease the rate

Wind Speed

Fan to increase wind speed

More wind speed, increase in rate