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Ecosystems of the Homewood Campus, Park V: Electrophoresis of PCR Products
Ecosystems of the Homewood Campus, Park V: Electrophoresis of PCR Products
General Notes
Gel Loading Due Purposes
Contains sucrose (or similar dense material like glycerol), which weighs down the sample so it sinks into the well
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Red is positive and black is negative. DNA is negatively charged so we want to set up the gel so that DNA flows towards red electrode
Part 1: Pour Agarose Gel
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Fit a comb into gel tray. Put the tray into electrophoresis box. Make sure the comb doesn't have old agarose gel pieces stuck to it
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Part 4: Stain Photograph, and Analyze Gel
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