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Sequenciamento de DNA (1975: Sanger dideoxy" chain-termination method…
Sequenciamento de DNA
1975
: Sanger dideoxy" chain-termination method (Mito, bacterio, humano)
time expensive
1993
: Pyrosequencing method
1998: apirase:single cost expensive
1997
: Ilumina
erros devido a pol.
1975
: Sanger plus and minus method
2005
:polony multiplex
2006
: SOLiD technology employs sequencing by ligation
2009
: ion torrent sequencing H+
2000
: 454 emulsion pyrosequencing
2010
: Nanoball+ SOLiD