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DNA: REPLICATION, TRANSCRIPTION AND TRANSLATION By: Maria Jose Andrade…

- - - - Technique called CAESIUM CHLORIDE DENSITY GRADIENT CENTRILUGATION.
        
        The gradient is established with the greatest caesium concentration and therefore density at the bottom and the lowest at the top of the tube.
        
        Any substance centrifuged with the caesium chloride solution becomes concentrated at a level corresponding with its density.
  - - - Unwinds the double helix
    - - The separation is carried out by helicases, a group of enzymes that use energy from ATP
        
        Energy of the ATP is used to move the helicase along the DNA molecule, breaking the hydrogen bonds between bases and parting the two strands.
  - - - Always moves along the template strand in the same direction, adding 1 nucleotide at the same time.
        
        Moves along the template strand, assembling the new with a base sequence complementary to the template strand.
        
        The assembly of the new strand is carried out by the enzyme DNA polymerase.
- - - - Ribosomes = small and large subunit.