Foodborn pathogen

BACTERIA

Campylobacter Jejuni

Morphology
-spiral- shape, non- spore forming.
-gram negative microaerophilic
-commonly found in animal traces.

Growth requirement
-best at 37-45°c

Symptoms
-abdominal pain😩, diarrhea(sometime bloody), : fever and nausea 😵

Diagnosis
-culture of stool specimen.

Mode of transmission
*consumption of contaminated food or water directly contact with infected animal including pets.

Clostridium Botulinum

Morphology
gram +ve, rod shape, anerobic. spore formation,.no time bacterium.

Diagnosis
Take blood, stool or gastric secretion sample.

Mode of transmission
*injection of inadequately cooked food or processed or refrigerated foods in which toxin particularly canned and alkaline foods.

Clostridium Perfringens

Symptoms
*double vision, droopy eyelids, trouble speaking and swallowing and difficult to breath.

Morphology
+ve, spore formation. anaerobic, rod shape bac that produce an enterotoxin which released by intes6.

Growth requirement.
*tem- 15-55°c, ph 5-9

Symptoms
*abdominal cramps, watery, diarrhea.

Diagnosis
stool specimen determine the num of bac in feces.

mode of transmission
-consuming large num of CP vegetative cell present in the food.

FUNGAI

MOLD

Monilia

Mucor

Gibberella

Penicillium

Aspergillus

Sclerotinia

YEAST

Debaryomyces

Kloeckera

False/ Wild yeast

PARASITE

Parasite Worms

Trematoda

Cestoda

Nematoda

Anisakis and pseudoterranova

Clonorchis/ Opisthorchis (liver flukes)

Taenia spp.

VIRUSES

Rotavirus

Norwalk virus

Hepatitis A virus

Escherichia coli 0157:H7

Symptoms
*diarrhea that often is bloody, nausea, fever (37.7- 38.3°c), loss of appetite and vomiting.

Mode of transmission
consuming contaminated water, raw milk, uncooked vegetables or fruits. person to person

Growth requirements
tem 4 - 45°c pH 3-6

Diagnosis
*

Morphology
-gram negative, rod shape (bacilli) bacterium, toxic forming aerobic.

Salmonella

Morphology
*gram negative, non -spore forming for.

Diagnosis
*stool specimen

Growth requirements
tem 4- 46°c pH 4.1 - 9.0

Mode of transmission
person to person or animal to person.

Symptoms
*stomach pain, diarrhea, nausea, fever, headache. Appear 6 to 48 hours.

Shigella

Staphylococcus Aureus

Streptococcus Aureus

Diagnosis: stool test.

Growth requirement: Tem- 10- 40 c, pH- below 4-5

Morphology: gram negative non spore forming, facultative anaerobic, rod shape bacteria.

Mode of transmission: contaminated food or water contect with infected objective or sexsual contect.

Symptoms: diarrhea, vomitting, abdominal pain, and fever.

Diagnosis: culture the bacteria

Growth requirement: Tem- 4 - 46 C, pH- 4.8-8.0

Morphology: gram positive, facultative aerobic spherical bacteria, produce a very heat stable toxin.

Mode of transmission: improper handing of food. food contaminated by human.

Symptoms: several abdominal pain, diarrhea, nausea dehydration and fever.

Diagnosis:culture the bacteria.

Growth requirement: Tem- 4 -46 C, pH- 4.8-8.0

Morphology: gram positive, appear grape like cluster,

Mode of transmission: improper handling of food and the food is contaminated by human.

Symptoms: several abdominal pain, diarrhea, nausea dehydration and fever.

Listeria Monocytogenes

Growth requirement: Tem- 45 - 1 c, pH- 4.5-9.6

Mode of transmission: direct contact with infections material or soil contaminated with infection animal feces.

Symptoms: abdominal pain, diarrhea, nausea, fever and headache.

Diagnosis: stool test.

Morphology: gram positive and rod shape bacteria.

Growth requirements.
*3-48°c , ph 4.6- 8.9

incubation: 10-50 days

Human is infected by eat or drink food or water that has been contaminated by feces, sexual practices oral-anal contact and does not wash hand properly after toilet and touch other object.

symptoms: sudden fever, jaundice, vomiting, abdominal discomfort, fatigue and bile in urine(dark urine).

incubation: 1-3 days.

inflammation of stomach and intestine.

Human infected by ingestion of contaminated food or water and direct contact with contaminated surface and putting hand in the mouth.

symptoms: fever, stomach cramps, dehydration, vomiting and diarrhea.

eating food or drinking water with contaminated with norovirus.

recovery: 12 - 60 hours usually.

symptoms: nausea, vomiting, diarrhea, anorexia, low grade fever and malaise.

Protozoan parasite

Giardia lambia

Toxoplasma gondi

diagnosis: blood test.

symptoms: fever, headache, muscle pain,sore throat, and swollen lymph node.

infected by consuming raw or uncooked meat, from a blood transfusion or organ transplant, transplacental transmission from mother to fetus and by ingesting contaminated water, soil or vegetable.

obligated, intracellular, parasite protozoan that causes toxoplasmosis.

Entamoeba histolytica

symptoms: abdominal pain, fever, severe diarrhea, and vomiting.

infect a person when ingest food or water has been contaminated within fected feces.

an anaerobic and cause Amoebiasis.

Cryptosporidium parvum

infected by, drinking untreated water,eating contaminated food.

some individual has no symptoms at all.

symptoms: watery diarrhea, stomach pain, dehydration, nausea, vomiting, fever,and weight loss.

cause Cryptosporidiosis, it is a diarrheal disease.

person to person.

cause Giardiasis

parasite that live in the intestine of animalor people.

frequent method of infection is by consuming contaminated food or water and also through hand-to-mouth transmission.

symptoms: abdominal cramp, diarrhea, gas and nausea.

found with fish.

treatment: physical removal of larva.

humans are accidental host and these larva cannot mature in human gut.

Ascaris Lumbricoides

intestine roundworm.

caused more severe infection in the liver or lungs.

ingest contaminated food and water.

diagnosis: stool sample.

lodge in the liver of infected human and other animal causing blockage and hyperplasia of the bile passages.

diagnosis: stool sample.

light infection can cause mild symptoms like liver dysfunction

heavy infection can cause symptoms like hepatitis and digestive disorders.

human infected by consuming contaminated food and water, direct hand, and fecally contaminated vegetable.

Taenia seginata (pork tapeworm) and Taenia solium (beef tapeworm)

symptoms: abdominal pain, diarrhea, altered appetite, and constipation.

diagnosis: stool specimen

caused by tapeworm.

when the larva reach brain cause neurocysticerosis

produce long,slender, pigmented, and conidiophores.

mycelium - septate

also refer to crown rot, stem rot, cottony rot, watery soft rot, drop and blossom blight.

able to produce black irregular sclerotia - cause grey mold condition on plant foods.

important cause of market disease of fruits and vegetables.

plant pathogenic fungus cause a disease called white mold.

produce extensive mycelium that is cottony in culture with tinges of pink, purple or yellow.

important in spoilage of of vegetable and fruits.

conidia: canoe shape,born single or in chains.

produce white or grey mycelia that bear branches.

conidia: pin or tan in mass

cause brown rot of fruits, red bread moulds.

Cephalothecium

produce mycelium that bear long,slender and simple conidiophores.

conidia: single, apically, sometime group of chain.

pink on vegetables and fruits.

produce non septate mycelium

the spore smooth,regular, and borne with sporangium.

found in large number of foods.

produce septate mycelium

color of food, blue to green-blue.

widespread in soil, dust, air and etc.

found in bread, cakes, fruits and preserves.

important in making cheese, and production of antibiotic (penicillin)

prduce septate mycelia, some aflatoxins, ochratoxin.

found in cake, fruits and vegetables.

appear yellow to green to black on foods.

conidia: one called, globose, and variously colored in mass

produce a pseudomycelium

do not display sexual reproduction

some are yeast like organisms along with common molds.

difficult to distinguish morphologically and culturally.

reproduce by multipolar budding

found in the surface of spoilage of food such as pickle, sausage, wine, fresh and cured meats.

non spore forming yeast.

common in fruits

possess both fermentative and oxidative abilities.

some cause of flavour and turbidity in wine.