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BIOINFORMATICS (How rDNA technology connected to Bioinformatics…
BIOINFORMATICS
Mapping of molecular biology knowlegde
Central Dogma
RNA
mRNA
RNA
Protein
Protein
phenotype
DNA
genomic sequence
RNA function & structure
protein sequence
protein structure
protein function
What Is Bioinformatics
application of computational tools on
molecular data
acquire, analyze, visualize
molecular data = sequence of DNA, cDNA or protein
Why do we use Bioinformatics
store/ retrive biological information (database)
predict function of unknoen sequence
How rDNA technology connected to Bioinformatics
PCR
amplified targeted DNA sequence using pair of primers
verify amplified DNA by AGE
DNA Sequencing
verify amplified DNA from PCR through DNA sequence
use automated DNA sequencing approach, result ouput in chromatogram
DNA Extraction
verify DNA through AGE
Bioinformatics
interpret message in DNA sequence
use BLAST programme provide by NCBI =>result shown in BLAST output
NCBl
= Natinal Centre for biotechnology Information
BLAST
= Basic Local Alignment Seacrch Tools
Identity vs Similarity
exact match between two nucleotides/ amino acid
resemblence between 2 residues that greater than 1 would expect in random