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(Why there is protein? (Key components of machinery ~ determine which…

- - - - cell disrupted to release protein.
    - - Use either mechanical or non-mechanical method
    - - Function
        
        To denature proteins completely
        
        To disrupt any disulfide bonds through reduction
        
        To dissolve any interfering particles
        
        To produce successful electrophoretic separation
      - Was carried out :question:
        
        in loading buffer
        
        Tris Cl ~ create stable environment for the proteins
        
        Glycerol ~ increase protein density, easier to load in the well
        
        SDS ~ to breakdown secondary structure of proteins, make negatively charged.
        
        B- Mercaptoethanol ~to breakdown disulfide bonds in tertiary structure
        
        Bromophenol blue ~ to track the run of protein sample on the gel
  - - - Migration of charged molecules in electric field
        to electrode opposite charges
      - treats samples with SDS to denature protein
      - Protein has unique comb. of amino acids, the net of charge is naturally different.
      - Intrinsic charges of protein = place strongly anionic detergent and SDS in sample and gel .
        
        SDS coats and binds to proteins and denatures them
      - Protein migrates in polycrylamide gel if equivalent negative charge densities and mass become variable affecting the migration.